Fig. 6.
Summary of apparent maximum growth rate (A) and biomass (B) produced at various nitrite concentrations for the growth of P. denitrificans PD1222 and the fhp mutant. Growth in minimal salts media, pH 7.5, supplemented with ammonium chloride, 10 mM, sodium succinate, 30 mM and Vishniac trace element solution. Measured by optical density at 600 nm (OD600nm) adjusted to a pathlength of 1 cm against time (h). Error bars denote standard error (n = 3<). (C). Growth of Paracoccus denitrificans PD1222 wild type (WT), FHP deletion mutant and FHP complement strain in minimal salts media, pH 7.5, supplemented with 50 mM sodium nitrite. Cells were grown in 100 μl total volume with shaking at 400 RPM. OD600nm was measured every 0.5 h, 1 h values shown. Error bars denote standard error n = 3.