Fig. 7.

Chemostat cultures of P. denitrificans cultured aerobically in the presence of nitrate. Continuous culture of Paracoccus denitrificans PD1222 under nitrosative stress with nitrite addition to minimal salts media at pH 7.5 and 10 mM ammonium chloride (n = 3). At 22 h feed was applied at a dilution rate of 0.05 h⁻¹ (vertical line at 22 h). Feed was replenished at 72 h (vertical line at 72 h). Top panel: average dissolved oxygen (DO), optical density (OD_600nm) and biomass. Lower panel: concentration of nitrate, nitrite and nitrous oxide throughout the incubation period. (Error bars denote standard deviation; n = 3).