Table 1.

Verification of the microarray analysis by qRT-PCR transcriptional analysis of selected genes of P. denitrificans grown aerobically at pH 7.5 in the presence of 12.5 mM nitrite.

Gene identifier (Pden_)	Gene name	Microarray transcription	qRT-PCR transcriptiona	±SD
3605	Acid stress	3.00	2.06	1.27
1690	BadM reg.	2.16	3.61	1.88
1129		0.21	0.43	0.25
5108	Cyt ba3	2.45	2.68	0.89
1629	sigma-24	0.45	0.39	0.13
3429	dctM	0.44	0.47	0.10
1689	fhp	2.17	3.43	0.37
4721	napA	0.89	0.38	0.10
4236	narG	1.72	0.84	0.15
2487	nirS	0.59	1.12	0.13
2483	norB	0.54	0.65	0.05
4452	nasB	0.93	1.18	0.14
4465	gapdha	1.18	0.98	0.04
0342	polB	1.12	1.03	0.04

±SD standard deviation n = 3 biologically independent replicates (additional 3 technical replicates).

^aAll qRT-PCR results use gapdh as housekeeping gene for normalisation, with the exception of gapdh itself whose transcription was verified with polB as housekeeping gene.