FIG. 3.
Immunoblot analysis of plasmid-expressed Lassa virus proteins. MVA-T7-infected BHK-21 cells were transfected with the constructs as indicated above the blots. Alternatively, Vero cells were infected with Lassa virus (only panel B). Negative control cells (NC) were infected with MVA-T7 and mock transfected. The background bands in the NC lane of panel C result from an overloading of the gel. The blots were incubated with the following antibodies: rabbit anti-Lassa Z protein (A), rabbit anti-Lassa NP (B), and anti-FLAG M2 monoclonal antibody (C and D).