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. 2004 Dec;78(24):13460–13469. doi: 10.1128/JVI.78.24.13460-13469.2004

FIG. 2.

FIG. 2.

(A) Schematic diagram of the pHEBo:Rp-Luc reporter plasmid and the EBNA1 expression plasmid puro/oriP-EBNA1. (B) Luciferase reporter assays following induction with anti-Ig. Rp+EBV and Zp+EBV were in AK2003 cells, and Rp and Zp were in EBV-negative AK31 cells. Co is the pHEBo-luc vector control in AK2003 cells. (C) Western blotting assay to detect induction of BZLF1 (BZ-1 antibody) or BRLF1 (8C12 antibody, 1:100) in EBV-negative AK31 cell lines containing p294:ZRp-254, p294:Zp-1276, or p294:ZRp-7101 compared to the positive control AK6 cells.