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. 2004 Dec;78(24):13460–13469. doi: 10.1128/JVI.78.24.13460-13469.2004

FIG. 5.

FIG. 5.

(A) Luciferase reporter assays for different classes of EBV promoter in AK2000 cells to show the relative timing after anti-Ig treatment and dependence of the late promoter on ori lyt. BZLF1, immediate early; BMRF1, delayed early; BcLF1, late; Cp, latent. Data are expressed as fold induction above the level of activity without anti-Ig treatment because of the different inherent strengths of the promoters. (B) All three of the late promoters tested in AK2000 cells require ori lyt for induction in response to anti-Ig and show late timing. (C) Core ori lyt reduces the lytic cycle activity of the delayed early promoter (BMRF1) in AK2000 cell lines and has no effect on the latent cycle promoter (Cp) in luciferase reporter assays after anti-Ig treatment. (D) Luciferase reporter assays with AK2000 cell lines for the BFRF3 and BcLF1 promoters with or without core ori lyt, compared to empty vector pHEBo-luc without ori lyt. Relative light unit (kRLU, 103 relative light units) data are shown as averages of duplicate determinations.