FIG. 6.
(A) Schematic diagram of the conserved −73 to −81 sequence present upstream of some late promoters and the mutation introduced. For the TATA box mutation, TATT was changed to TATA. (B) Luciferase reporter assays of the late promoter for BcLF1 in response to anti-Ig treatment in AK2000 cells. 5′ deletions were to position −430, −109, or −49, and −109m has the −81 to −73 sequence mutated in the −109 background. (C) Similar to panel B but with the late promoter for BdRF1. (D) TATT-to-TATA mutations of the TATA box in promoters for BdRF1 and BcLF1 in the −109 plasmids. kRLU, 103 relative light units; wt, wild type.