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. 2004 Dec;78(24):13943–13953. doi: 10.1128/JVI.78.24.13943-13953.2004

FIG. 3.

FIG. 3.

Effect of overexpression of full-length LDI-1 (LDI-1 FL) or a HECT domain mutant of LDI-1 (LDI-1 FL C/A) on release of RSV Gag from 293/E cells. (A) RSV Gag was expressed in 293/E cells in the absence or presence of increasing amounts of p2036-LDI-1 FL. The amounts of Gag in the lysate and medium fractions were determined as described in Materials and Methods. Untransfected cells, lane 1; cells transfected with 0.5 μg of p2036-Gag, lane 2; cells cotransfected with 0.5 μg of p2036-Gag and 0.5 μg of p2036-LDI-1 FL (lane 3); cells cotransfected with 0.5 μg of p2036-Gag and 1 μg of p2036-LDI-1 FL (lane 4); or cells cotransfected with 0.5 μg of p2036-Gag and 1.5 μg of p2036-LDI-1 FL (lane 5). (B) Graphical representation of relative amounts of Gag released into the medium in the presence of LDI-1 FL (derived from two independent experiments). (C) RSV Gag was expressed in 293/E cells in the absence or presence of increasing amounts of p2036-LDI-1 FL C/A. Untransfected cells, lane 1; cells transfected with 0.5 μg of p2036-Gag, lane 2; cells cotransfected with 0.5 μg of p2036-Gag and 0.5 μg of p2036-LDI-1 FL C/A, lane 3; cells cotransfected with 0.5 μg of p2036-Gag and 1.5 μg of p2036-LDI-1 FL C/A, lane 4. (D) Graphical representation of relative amounts of Gag released into the medium in the presence of LDI-1 FL C/A (derived from two independent experiments).