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. 2017 Jan 3;31(3):755–759. doi: 10.1038/leu.2016.341

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Copyright © 2017 The Author(s)

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Impact of UNBS1450 alone or with ABTs. (a) Effect of UNBS1450 and ABT-199 single/combination treatments on tumor formation in a zebrafish xenograft model with U937 cells injected after an 8 h UNBS1450/ABT-199 pretreatment. Analysis of differential toxicity in healthy donor CD34⁺ (b) and platelets (c) by Annexin-V (BD Pharmingen, Erembodegem, Belgium) and Cell Titer Glo assays (Promega, Leiden, The Netherlands), respectively. Apoptogenic potential of UNBS1450 alone or in combination on AML patient samples (Supplementary Data): (d) analysis of UNBS1450 alone (by Annexin-V assay or MitoTracker Red staining (Invitrogen, Thermo Fisher Scientific, Asse, Belgium)); (e) combination of UNBS1450 (30 nm; 48 h preincubation) and ABT-199 (0.01 μm; 18 h of incubation). (f) Same analysis in AML patient lymphocytic subpopulation. (g) Western blot analysis of anti-apoptotic Bcl-2 protein expression. Synergy was estimated by using the ‘response additivity' approach. Corresponding combinational index (CI) of significant interactions were computed. Statistical analyses were performed in GraphPad Prism (GraphPad Software Inc., La Jolla, CA, USA). Significance is *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 (two-way analysis of variance; repeated measures; post hoc analyses Dunnett; Sidak).