TABLE 3.
MAb reactivities for wild-type and mutant plasmid-transfected cells and for secreted VLP antigens
| Strain or assaya | Epitope, MAb, PRNT, SA, specificityb
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Polyclonal, D2HIAE, +, NA | A1a, 4G2, +, +, group | A1b, 6B6C-1, ±, +, group | A2, 4E5, +, −, subcomplex | A3, 1A5D-1, −, ±, type | A5, 1B7-5, +, +, subgroup | A/C?, 10A1D-2, −, ±, complex | C1, 1B4C-2, −, +, subcomplex | C4, 9A4D-1, −, −, type | B1, 3H5, +, +, type | B2, 9A3D-8, +, +, type | B3, 10A4D-2, +, +, subcomplex | B4, 9D12, +, +, type | |
| Wild Type | |||||||||||||
| IFA | 4.1 | 3.8 | 3.8 | 2.6 | 4.4 | 4.1 | ≥2.9 | 4.4 | ≥2.9 | >4.4 | 3.5 | 4.1 | >4.4 |
| Ag-ELISA | >6.0 | >6.0 | ≥6.0 | ≥2.9 | 4.2 | 5.7 | >3.8 | >5.3 | 2.9 | >6.0 | >6.0 | >6.0 | >6.0 |
| T76M | |||||||||||||
| IFA | ND | ND | |||||||||||
| Ag-ELISA | ≤5 | 0.8 | |||||||||||
| G104H | |||||||||||||
| IFA | <3 | 6 | <0.8 | 3 | ND | ND | |||||||
| Ag-ELISA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| G106Q | |||||||||||||
| IFA | <3 | <3 | <0.8 | ND | 6 | ND | |||||||
| Ag-ELISA | <0.1 | <0.1 | <6 | 13 | ≤0.1 | ||||||||
| L107K | |||||||||||||
| IFA | <3 | <25 | 6 | ND | ND | ||||||||
| Ag-ELISA | <0.1 | 5 | 6 | 0.2 | |||||||||
| E126A | |||||||||||||
| IFA | 6 | ND | ND | ||||||||||
| Ag-ELISA | 10 | 13 | |||||||||||
| E126A/T226N | |||||||||||||
| IFA | 3 | 3 | <25 | ND | ND | ||||||||
| Ag-ELISA | 5 | 13 | |||||||||||
| W231F/L | |||||||||||||
| IFA | <3 | <3 | <2 | 6 | |||||||||
| Ag-ELISA | NA | NA | NA | NA | NA | NA | |||||||
Reciprocal end-point titers (log10) for plasmid-transfected cells (IFA) and for secreted VLPs (Ag-ELISA) are shown for the wild-type non-mutated plasmid. MAb end-point values for mutant plasmids and mutant VLP antigen that differed more than fourfold from those of the non-mutated wild-type plasmid are shown as percentages of the remaining wild-type antigen reactivity and shown in bold. MAb end-point values that did not differ from the wild-type by four-fold or more were left blank. NA, not applicable (these constructs did not secrete VLP antigen and thus could not be examined by Ag-ELISA); ND, not determined.
PRNT, plaque reduction neutralization titer at a 1:100 dilution of ascitic fluid (46); SA, epitope surface accessibility from antigen-capture log10 ELISA end-point titers. −, ≤3.0; ±, 3.0 to 5.0; +, ≥5.0.