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. 2016 Nov 4;31(3):645–653. doi: 10.1038/leu.2016.258

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Commd1 and Cullin1 regulation of p27. (a) Western blot of p27 from lysates after transfection with scrambled, COMMD1 or CUL1 shRNA. Cell lysates were prepared as previously described, separated by SDS–polyacrylamide gel electrophoresis and probed with indicated antibodies.⁴⁰ (b) Association of Commd1 with HA-tagged Cullin-1. Western blot of HA immunoprecipitates from PI-sensitive or -resistant RPMI826 or U266 cells transfected with a plasmid that expressed HA-CUL-1 after PI treatment. Lysates were immunoprecipitated with IgG as controls (lanes 1 and 2).