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. 2004 Dec;78(24):13420–13429. doi: 10.1128/JVI.78.24.13420-13429.2004

FIG. 7.

FIG. 7.

Requirement for compatible interaction between 5′ and 3′ NCR in BMV RNA2. A Northern blot examining the accumulation of the minus-strand replication products from several chimeric BMV RNAs is shown. The names of the RNAs denote the origin of the 5′ NCR, the protein-coding region, and the 3′ NCR of a BMV RNA. For example, R2/1/2 indicates that the 5′ and 3′ NCR came from RNA2 but that the protein-coding region was from RNA1. Plus-strand RNA products are not shown but exhibited the same trends seen with the minus-strand RNAs. The combinations of the RNAs transfected into protoplasts are shown above the gel image. Total RNAs were purified from protoplasts at the times (in hours) indicated below the gel images. Asterisks identify the positions expected of the chimeric RNAs. All of the samples in this experiment were on one blot but were cropped to present the results in a more logical manner.