Table 1.

The analysis of tsetse fly samples using T. brucei gambiense standard and stem LAMP test formats.

Source	Number of poolsa	Template type	LAMP tests
			Standard LAMPb	C T  valuec	Stem LAMPw	C T value	Stem LAMPwn	C T value
Midgut	5	DNA	+	26	+	19	+	19
		Supernatant	+	23	+	15	+	15
	10	DNA	−	—	+	21	+	21
		Supernatant	+	25	+	18	+	18
	15	DNA	−	—	−	—	−	—
		Supernatant	−	—	+	24	+	24
	20	DNA	−	—	−	—	−	—
		Supernatant	−	—	−	—	−	—
Whole fly	1	DNA	+	28	+	20	+	20
		Supernatant	+	26	+	19	+	20
	5	DNA	−	—	−	—	−	—
		Supernatant	−	—	+	—	+	23
	10	DNA	−	—	−	—	−	—
		Supernatant	−	—	−	—	+	28†

^aInsectary G. pallidipes (midguts or whole fly).

^bPublished TgsGP LAMP test [24].

^cCycle threshold (minutes).

^†2 out of 6 replicates showed the predicted product.