TABLE 4.
Genetic and phenotypic characterizations of type 1 cVDPV in the Philippines
| Virus | Nucleotide and amino acid reversionsa
|
Neutralization titer
|
Neuro- virulencec (PD50) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 5′-NTR at nt:
|
Capsid
|
Nonstructural proteins
|
3′-NTR at nt:
|
|||||||||||||||
| 26 | 355 | 480 | VP4-65 | VP1-99b | VP1-106b | 2A-134 | 2B-95 | 3D-53 | 3D-73 | 3D-250 | 3D-362 | 7410 | 7441 | 8a034 | 8a057 | 11m071 | ||
| Sabin 1 | G | T | G | S | K | T | T | T | N | H | E | I | C | G | 102,400 | >64,000 | <20 | >8.0 |
| Mindanao-01-1 | A | C | A | A | T | A | S | I | D | Y | K | T | U | A | <20 | 673 | 905 | 2.4 |
| Luzon-01-1 | A | C | A | A | T | A | S | I | D | Y | K | T | U | A | <20 | 800 | 1,810 | ND |
| Luzon-01-2 | A | C | A | A | T | A | S | I | D | Y | K | T | U | A | <20 | 336 | 1,076 | 2.6 |
| Luzon-01-2c | A | C | A | A | T | A | S | I | D | Y | K | T | U | A | ND | ND | ND | 4.9 |
| Mahoney | A | C | A | A | T | A | S | I | D | Y | K | T | U | A | <20 | 450 | 160 | 2.6 |
| HAI00-003d | A | C | A | A | T | A | A | I | D | Y | K | T | U | A | ND | ND | ND | 2.8 |
a
Nucleotide and amino acid reversions from the Sabin 1 (Genbank accession no. V01150) to Mahoney (VO1149; the neurovirulent parent of Sabin 1) sequences are indicated. Nucleotide substitutions are given only for the 5′- and 3′-NTRs.
b
Surface residues forming part of neutralizing antigenic site 1.
c
ND, not determined.
d
A representative type 1 cVDPV strain from Hispaniola. Its PD50 value is quoted from a previous report (25).