Figure 3.

Effect of previous immunization with pVAXhsp65 on CNS inflammation. C57BL/6 mice were immunized with 4 pVAXhsp65 doses and then submitted to EAE induction. Lumbar spinal cord inflammatory infiltrates in control mice without EAE (a), control mice with EAE (b), and mice immunized with 4 pVAX (c) or pVAXhsp65 doses (d) were evaluated 30 days after EAE induction. A semiquantitative analysis was used to assess the inflammatory infiltration (e) during the chronic disease phase. Micrographs are representative of 6 animals/group. Data in panel (e) were presented by mean ± SE of 6 mice. ∗ represents the difference between immunized and control group with EAE. p < 0.05.