TABLE 2.
Titration of infection by biological and molecular PERV isolatesa
| Cell line (species) | Titer (IU/ml)
|
|||
|---|---|---|---|---|
| PERV-A 14/220 | A14/220 | PERV-A PK | Ap60 | |
| Replicating PERVb | ||||
| 293T (human) | 2,600,000 | 1,800,000 | 42,120 | 37,000 |
| Mv-1-Lu (mink) | 130,000 | 180,000 | 5,900 | 4,500 |
| CRFK (cat) | 410,000 | 170,000 | 17,000 | 12,000 |
| LacZ pseudotype | ||||
| 293T (human) | 15,000 | 21,000 | 1,200 | 420 |
| TE671 (human) | 11,000 | 77,000 | 1,400 | 930 |
| Pindak (squirrel monkey) | 1,500 | 1,300 | <10 | <10 |
| Mv-1-Lu (mink) | 17,000 | 76,000 | 1,000 | 1,000 |
| CRFK (cat) | 7,700 | 7,600 | 120 | 450 |
| ST-IOWA (pig) | 26,000 | 37,000 | 2,500 | 2,500 |
a
Biological isolates, PERV-A 14/220 and PK, and viruses derived from molecular clones, A14/220 and Ap60, were titrated. A data set representative of two independent experiments is shown.
b
Titration for replicating PERV was not possible because of high background in the immunostaining assay in TE671, Pindak, and ST-IOWA cells.