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. 2017 Mar 6;149(3):355–372. doi: 10.1085/jgp.201611664

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© 2017 Zhang and Hwang

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Figure 3. — O₁O₂ phenotype revealed by charge displacement in TM6. (A) A representative recording of R352Q barely shows two conductance levels because the single-channel amplitudes of both the O₁ state (0.09 ± 0.01, n = 4) and the O₂ state (0.13 ± 0.01, n = 4) are dramatically reduced because of the neutralization of arginine 352 in the internal vestibule of CFTR. The two peaks in the all-points histogram on the right are mostly coalesced into a single peak. (B) A representative recording of the double mutant M348R/R352Q also displays the O₁O₂ phenotype with much higher single-channel amplitudes for both the O₁ state (0.17 ± 0.01, n = 4) and the O₂ state (0.25 ± 0.01, n = 4) as measured in the all-points histogram on the right. (C) A representative recording of M348R with a single-channel amplitude of 0.35 ± 0.01 (n = 4) and the corresponding all-points histogram on the right.