TABLE 3.

Motif-based CD8 T-cell epitope prediction for the PEVKEK region of CVB4 E2 and homologous region of GAD₆₅^a

Antigen	Sequence no.	Sequence	Binding score
			A2.1	B8
CVB4-E2 polyprotein	1133	KILPEVKEK	58	55
	1135	LPEVKEKHE	32	100
	1137	EVKEKHEFL	64	220
GAD65	257	KMFPEVKEK	52	50
	259	FPEVKEKGM	33	95
	261	EVKEKGMAA	46	165

^aClass I epitope prediction using the HLA Ligand/Motif database (http://hlaligand.ouhsc.edu/LigandDB/servlet/GenerateFormServlet?form_type=index), is shown. For each peptide, the binding score represents the sum of each of the individual scores of the nine amino acid positions of the peptide binding groove. For each position of the peptide binding groove of a certain HLA allele, the percentage of all the amino acids is recorded, and based on this percentage, all the residues are scored for each position. Similar results were obtained with the SYFPEITHI database (http://www.uni-tuebingen.de/uni/kxi/).