Table 2.
Efficiency values and correlation coefficient for each candidate reference genes under evaluation.
| Gene | RT-qPCR efficiency% (R2) | ||
|---|---|---|---|
| CL153 | Icatu | IPR108 | |
| GAPDH | 93.3(0.99) | 95.7(0.98) | 93.9(0.98) |
| EF-1A | 96.3(0.99) | 82.7(0.99) | 97.3(0.96) |
| ELF-4A | 90.8(0.99) | 90.9(0.98) | 93.3(0.96) |
| CYCL | 87.1(0.96) | 80.3(0.98) | 80.3(0.98) |
| ACT | 91.4(0.93) | 98.7(0.93) | 97.9(0.91) |
| DNAJ | 82.5(0.99) | 86.4(0.99) | 97.7(0.98) |
| S15 | 99.9(0.96) | 98.8(0.98) | 90.0(0.95) |
| MDH | 80.3(0.99) | 92.0(0.96) | 92.1(0.97) |
| a-TUB | 99.3(0.96) | 93.4(0.98) | 94.0(0.99) |
| UBQ2 | 98.1(0.97) | 91.2(0.93) | 92.2(0.94) |