Table 6.

Analysis of RLS expression by RT-qPCR, calibrated with the two most (MDH and UBQ2) and the two least (a-TUB and CYCL) genes for the temperature group/treatment.

Reference genes			MDH and UBQ2	a-TUB and CYCL
Genotype	Temperature	[CO2]	RLS expression	RLS expression
CL 153	25/20°C	380	1.00	1.00
		700	0.361	1.768
	31/25°C	380	1.353	5.665
		700	0.504	3.559
	37/30°C	380	1.581	12.556
		700	1.467	12.86
	42/34°C	380	4.565	25.922
		700	8.889	25.114
Icatu	25/20°C	380	1.00	1.00
		700	0.781	1.553
	31/25°C	380	1.900**	3.604*
		700	1.505	2.546*
	37/30°C	380	3.423*	6.957*
		700	2.776*	5.742*
	42/34°C	380	5.220*	19.923*
		700	7.097*	32.153*
IPR 108	25/20°C	380	1.00	1.00
		700	0.249*	0.603
	31/25°C	380	0.778	1.345
		700	0.801*	1.720*
	37/30°C	380	0.844	1.851***
		700	1.070	2.172
	42/34°C	380	4.940*	21.732**
		700	1.399	4.01

For sake of simplicity, only significant differences were indicated (

^***P < 0.001;

^**P < 0.01;

^*P < 0.05).