Table 7.

Analysis of RLS expression by RT-qPCR, calibrated with the three most (MDH, UBQ2 and DNAJ) and the three least (a-TUB, CYCL and EF-1A) genes for the temperature group/treatment.

Reference genes			MDH, UBQ2, and DNAJ	α-TUB, CYCL and EF-1A
Genotype	Temperature	[CO2]	RLS expression	RLS expression
CL 153	25/20°C	380	1.00	1.00
		700	0.410	1,347
	31/25°C	380	1,424	2,780
		700	0.522	1,747
	37/30°C	380	1,730	5,701
		700	1,435	6,357
	42/34°C	380	4,214	10,853
		700	6,527	10,782
Icatu	25/20°C	380	1.00	1.00
		700	0.744	1, 449
	31/25°C	380	1.608*	2.807**
		700	1,215	2.170*
	37/30°C	380	2.726*	5.250*
		700	2.219*	3.955*
	42/34°C	380	4.087*	9.637*
		700	5.389*	12.979*
IPR 108	25/20°C	380	1.00	1.00
		700	0.249*	1, 059
	31/25°C	380	0.697*	1.307*
		700	1,123	2.865*
	37/30°C	380	0.759	1,495
		700	1, 093	3,453
	42/34°C	380	4.950*	11.012*
		700	1,759	4,645

For sake of simplicity, only significant differences were indicated (

^***P < 0.001;

^**P < 0.01;

^*P < 0.05).