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. 2004 Dec;24(24):10792–10801. doi: 10.1128/MCB.24.24.10792-10801.2004

FIG. 2.

FIG. 2.

GSE-Tid1 promotes immortalization of primary REFs. (A) GSE-Tid1 corresponds to the 3′ end of the cDNA for the short splice variant of mouse Tid1. (B) Protein expression analysis using Tid1 (L+S)-specific antibody PAbRS13 or Tid1S-specific antibody PAbS9 revealed changes in protein level and representation of isoforms in cells expressing GSE-Tid1 compared to those of cells immortalized with other GSEs. (C) GSE-Tid1 promotes immortalization of REFs with HPV E7 and cooperates with oncogenic ras for transformation. P2 REFs were transfected with either pLNCX (2.5 μg), GSE-Tid1 (2.5 μg), or GSE-p53 (2.5 μg) in conjunction with ras (2.5 μg) or HPV E7 (1 μg) or in triple transfections with both HPV E7 and ras. GSE-p53, the positive control, produced the highest level of immortalization in all cotransfection experiments. (D) GSE-Tid1 promotes immortalization of REFs with SV40 T antigen (Tag). REFs (passage 2) were transfected with pLNCX (2.5 μg) or GSE-Tid1 (2.5 μg) either alone or with pSE (SV40 whole early region, 2.5 μg; Tag wt). 3′UTR, 3′ untranslated region.