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. 2004 Dec;24(24):10593–10610. doi: 10.1128/MCB.24.24.10593-10610.2004

FIG. 1.

FIG. 1.

p73 colocalizes with PIAS-1. (A) Schematic representation of the p73 constructs used in this study. Boxes indicate the different functional domains: transactivation domain (TA) in red, DNA binding domain (DBD) in yellow, oligomerization domain (OD) in green, and sterile alpha motif (SAM) in pink. The fragment of p73 used as bait in the yeast two-hybrid screening is also shown. The asterisk indicates the position of lysine 627, where sumoylation occurs. (B) Schematic representation of the PIAS-1 constructs used in this study. Boxes indicate the different functional domains: SAF-AB Acinus and Pias domain in orange, PINIT domain (essential for nuclear retention) (7) in blue, zinc finger-like RING domain in green, and serine/threonine rich region (34) in purple. The asterisk indicates the position of the catalytic cysteine at position 350. (C) p73 and PIAS-1 colocalize in the nucleus when overexpressed in Saos-2 cells. An HA-TAp73α expression vector and a Flag-PIAS-1 expression vector were transiently transfected in Saos-2 cells alone or in combination. At 24 h after transfection, cells were fixed and stained with HA polyclonal (Y-11) and anti-Flag monoclonal (M2) antibodies.