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. 2017 Feb;20(2):122–130. doi: 10.22038/ijbms.2017.8231

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Copyright: © Iranian Journal of Basic Medical Sciences

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Purification and expression of the recombinant CFP-10:Fcγ2a and CFP-10:His fusion proteins. A) Analysis of the recombinant CFP-10:Fcγ2a protein by 12% SDS-PAGE stained with Coomassie Blue. Lane 1, 2: fractions of the eluted recombinant protein of approximately 35 kDa. Lane M: protein marker. B) The expression of the recombinant CFP-10:Fcγ2a protein was analyzed by western blot using anti mouse IgG-HRP. C) Analysis of the recombinant CFP-10:His protein by 12% SDS-PAGE stained with Coomassie Blue. Lane 1: fractions of the eluted recombinant protein of approximately 13 kDa. Lane M: protein marker. D) The expression of the recombinant CFP-10:His protein was analyzed by Western blot using anti His tag-HRP. Lane M: protein marker