Figure 8.

Fluorescent staining for mRNA damage in (A) control-sham, (B) 0.2 mg/kg nicotine-received and (C) 0.4 mg/kg nicotine-received groups. The cross section from control-sham animals is representing intact cells with normal mRNA with red-fluorescent appearance (Arrows). However, note a significant enhancement in mRNA damage at germinal cell levels in nicotine-received animals (yellowish-fluorescent, thick arrows). (D) semi-quantitative analyze for mRNA and (E) percentage of tubules with mRNA damage, all data are presented in Mean±SD, * versus **, (P<0.001)