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. 2017 Mar 6;214(3):681–697. doi: 10.1084/jem.20160446

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© 2017 Ercan et al.

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Figure 3. — CM collected from Tsc2 KD neurons blocks the maturation of wild-type oligodendrocytes. (A) Schematic cartoon of oligodendrocyte differentiation, showing OPC and developing and mature/myelinating oligodendrocyte (OL) stages. The stage-specific markers are displayed in parentheses. OPCs, which are treated by either control KD CM or Tsc2 KD CM are stained for different stage-specific markers as shown. (B) The cells are stained for A2B5 (green) and Hoechst (blue). (C) The graph shows the quantification of A2B5⁺ cells. (D) The cells are stained for O4 (green) and Hoechst (blue). (E) The graph shows the quantification of O4⁺ cells. (F) The cells are stained for MBP (green) and Hoechst (blue). (G) The graph shows the quantification of MBP⁺ cells. Quantifications of cells normalized (Norm.) to Hoechst⁺ cells (total cell number) and to the cells treated with CM from noninfected neurons are shown. The error bars represent SEM. *, P < 0.05; Student’s t test. n = 3 independent experiments were performed for all. Bars, 100 µm.