FIG. 5.

CRM1 inhibition does not alter cardiomyocyte viability. (A) NRVMs were left untreated or stimulated with PE (20 μM) for 48 h in the absence or presence of the indicated concentrations of LMB. Cells were stained with calcein AM dye, which is retained by and fluoresces in viable cells (green), and ethidium bromide (red), which enters only cells with compromised membranes. (B) AK was detected in culture medium of NRVMs stimulated for 48 h with PE (20 μM) or FBS (5%) in the absence or presence of LMB (18.5 nM). Values represent the means ± standard deviations of results from eight independent samples. The higher values from FBS-treated cells are a consequence of the AK present in serum. (C) NRVMs were cultured in 96-well dishes and treated with the indicated concentrations of LMB for 48 h in the absence of hypertrophic stimulus. Intracellular ATP concentrations were determined for eight independent samples from each treatment group and are graphed relative to values for untreated cells (set at 100). Standard deviations are shown.