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. 2017 Mar 6;214(3):753–771. doi: 10.1084/jem.20162089

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© 2017 Stanley et al.

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Figure 1. — Nol3 deletion leads to peripheral cytopenias and extramedullary hematopoiesis. (A) Hemoglobin (Hb), platelet count (PLT), monocyte, neutrophil, and lymphocyte percentage in the peripheral blood of Nol3^+/+ and Nol3^−/− mice (Nol3^+/+, n = 20; Nol3^−/−, n = 25). (B) Representative images of spleens from Nol3^+/+ and Nol3^−/− mice. (C) Spleen weights of Nol3^+/+ and Nol3^−/− mice (Nol3^+/+, n = 23; Nol3^−/−, n = 29). (D) H&E staining of Nol3^+/+ and Nol3^−/− spleens. White arrow highlights megakaryocyte clustering in Nol3^−/− spleens. Bars: (left) 400 µm; (middle) 80 µm; (right) 10 µm. (E) Representative images of myeloperoxidase staining of Nol3^+/+ and Nol3^−/− spleens. Bars: (left) 1,000 µm; (right) 200 µm. (F and G) Representative histogram plots and quantification of Hoechst-stained spleen cells for cell cycle analysis of Nol3^+/+ and Nol3^−/− spleens (Nol3^+/+, n = 7; Nol3^−/−, n = 5). (H) Total cell numbers of mature cells of different lineages in Nol3^+/+ and Nol3^−/− spleens (Nol3^+/+, n = 6; Nol3^−/− < 700 mg, spleen, n = 5; Nol3^−/− > 700 mg, spleen, n = 3). (I) Total cell numbers of LK and LSK cells in Nol3^+/+ and Nol3^−/− spleens (Nol3^+/+ n = 7, Nol3^−/− < 700 mg spleen weight n = 4, Nol3^−/− > 700 mg spleen weight n = 2). Bars represent mean values. Error bars represent ±SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001.