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. 2017 Mar 6;214(3):753–771. doi: 10.1084/jem.20162089

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© 2017 Stanley et al.

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Figure 2. — Increased proliferation and myelofibrosis in Nol3^−/− bone marrow. (A) Representative femurs from Nol3^+/+ and Nol3^−/− mice. (B) Total number of cells in Nol3^+/+ and Nol3^−/− bone marrow (Nol3^+/+, n = 17; Nol3^−/−, n = 10). (C) Absolute number of mature cell types in Nol3^+/+ and Nol3^−/− bone marrow (Nol3^+/+, n = 4; Nol3^−/−, n = 5). (D) H&E staining of Nol3^+/+ and Nol3^−/− bone marrow sections. Arrows highlight blood sinusoids present in Nol3^+/+ femurs. Bars: (left) 1,000 µm; (middle) 200 µm; (right) 80 µm. (E) Reticulin staining of Nol3^+/+ and Nol3^−/− bone marrow sections. Bars: (left) 200 µm; (right) 80 µm. (F and G) Representative histogram plots and quantification of Hoechst stained bone marrow cells for cell cycle analysis of Nol3^+/+ and Nol3^−/− bone marrow (Nol3^+/+, n = 7; Nol3^−/−, n = 5). (H) Percentage of live and apoptotic cells in the bone marrow of Nol3^+/+ and Nol3^−/− mice measured by Annexin V and DAPI staining (n = 6). (I) Peripheral blood colony assay of Nol3^+/+ and Nol3^−/− MPN mice (Nol3^+/+, n = 5; Nol3^−/− MPN, n = 4). (J) Cytokine measurements from pooled serum samples of Nol3^+/+ or Nol3^−/− mice (n = 6). Bars represent mean values. Error bars represent ±SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001.