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. 2017 Mar 6;214(3):753–771. doi: 10.1084/jem.20162089

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© 2017 Stanley et al.

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Figure 3. — Nol3^−/−-induced MPN is cell intrinsic and transplantable into congenic recipients. (A) Peripheral blood counts of mice transplanted with either Nol3^+/+ or Nol3^−/− MPN whole bone marrow cells. Hemoglobin (Hb), platelet (PLT), and monocyte counts are shown (n = 4). (B and C) Representative spleen and bone marrow images of recipient mice after transplantation with Nol3^+/+ or Nol3^−/− MPN total bone marrow cells. (D) Spleen weight of mice transplanted with either Nol3^+/+ or Nol3^−/− MPN cells (n = 4). (E) Representative H&E of bone marrow sections from congenic recipient mice after transplantation with Nol3^+/+ or Nol3^−/− MPN whole bone marrow cells. Bars: (left) 400 µm; (right) 200 µm. (F) Representative reticulin staining of bone marrow sections from congenic recipient mice after transplantation with Nol3^+/+ or Nol3^−/− MPN whole bone marrow cells. Bars: (left) 80 µm; (right) 10 µm. (G) Representative H&E staining of spleen sections from congenic recipient mice after transplantation with Nol3^+/+ or Nol3^−/− MPN cells. White arrows highlight megakaryocyte clustering in mice transplanted with Nol3-deficient cells. Bars: (left) 200 µm; (right) 80 µm. (H) Images of spleen and femoral bones of congenic recipient mice after transplantation with Nol3^+/+ and Nol3^−/− MPN total spleen cells. (I) Percentage of peripheral blood donor cell engraftment at 12 and >20 wk after competitive transplantation of either Nol3^+/+ or Nol3^−/− cells with wild-type competitor cells (Nol3^+/+, n = 6; Nol3^−/− MPN, n = 5). Bars represent mean values. Error bars represent ±SD; *, P < 0.05; **, P < 0.01.