Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 6;214(3):753–771. doi: 10.1084/jem.20162089

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Stanley et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Expanded Thy1⁺LSK cells exhibit increased cycling and myeloid differentiation bias in Nol3^−/− MPN. (A) Absolute numbers of MEP, CMP, and GMP cells in bone marrow of Nol3^+/+ and Nol3^−/− MPN mice (Nol3^+/+, n = 7; Nol3^−/− MPN, n = 6). (B) Absolute numbers of MPP, ST-HSC, LT-HSC, and Thy1⁺LSK cells in bone marrow of Nol3^+/+ and Nol3^−/− MPN mice (Nol3^+/+, n = 7; Nol3^−/− MPN, n = 6). (C) Total cell number of MPP, ST-HSC, LT-HSC, and Thy1⁺LSK in spleens of Nol3^+/+ and Nol3^−/− MPN mice (Nol3^+/+, n = 7; Nol3^−/− MPN < 700 mg, spleen, n = 4; Nol3^−/− MPN > 700 mg, spleen, n = 2). (D and E) Representative FACS plots and quantification of the cell cycle status of Nol3^+/+ and Nol3^−/− MPN Thy1⁺LSK bone marrow cells based on Ki-67 and Hoechst staining (Nol3^+/+, n = 7; Nol3^−/− MPN, n = 8). (F–H) Percentage of live and apoptotic cells in bone marrow LK, LSK, and Thy1⁺LSK cells, respectively, from Nol3^+/+ and Nol3^−/− MPN mice measured by Annexin V and DAPI staining (n = 3). (I) Colony-forming capacity of sorted Nol3^+/+ and Nol3^−/− MPN Thy1⁺LSK (Nol3^+/+, n = 6; Nol3^−/− MPN, n = 3). (J) Representative FACS plots of Gr-1 and CD11b staining of colonies derived from Nol3^+/+ and Nol3^−/− MPN cells. (K) Percentage of Gr-1⁺CD11⁺ and Gr-1⁻CD11b⁺ cells after colony formation assay of Nol3^+/+ and Nol3^−/− MPN cells (n = 3). (L) Wright-Giemsa staining of cytospun cells after colony assay of Nol3^+/+ and Nol3^−/− MPN Thy1⁺LSK cells. Bars, 80 µm. Bars represent mean values. Error bars represent ±SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001.