Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 6;214(3):753–771. doi: 10.1084/jem.20162089

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Stanley et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 9. — Nol3-deficient Thy1⁺LSK cells have a molecular phenotype similar to human PMF, and NOL3 is deleted and down-regulated in patients with myeloid malignancies. (A) Waterfall plot of NOL3 expression in CD34⁺ cells from 41 patients with PMF relative to the mean expression of NOL3 in CD34⁺ cells from 16 healthy controls (GEO accession no. GSE53482). (B) Box plot summary of NOL3 expression in CD34⁺ cells from patients with PMF in comparison to CD34⁺ cells from healthy controls (GSE53482). (C) Waterfall plot of relative expression of NOL3 in CD34⁺ cells from 41 patients with PMF (same data as in A) with indicated JAK2V617F mutation status relative to the mean expression of NOL3 in CD34⁺ cells from 16 healthy controls (GSE53482). (D) Box plot summary of NOL3 expression in CD34⁺ cells from patients with PMF with or without JAK2V617F mutations in comparison to CD34⁺ cells from healthy donors (GSE53482). (E) Comparative GSEA analysis depicting significantly positively enriched gene sets (FDR q-value < 0.01) in Nol3^−/− MPN (vs. Nol3^+/+) Thy1⁺LSK cells and CD34⁺ cells from PMF patients (vs. healthy controls). P < 2.2 × 10⁻¹⁶; odds ratio, 28.19. (F) Comparative GSEA analysis depicting significantly positively enriched gene sets (FDR q-value < 0.01) in Nol3^−/− MPN (vs. Nol3^+/+) Thy1⁺LSK cells and CD34⁺ cells from PMF patients dichotomized by high and low NOL3 expression. P < 2.2 × 10⁻¹⁶; odds ratio, 27.99. (G) Commonly deleted region (hg18) on chromosome 16 in human patients with myeloid malignancies. Yellow, NOL3 locus; pink bars, length of region with UPD; green bars, length of chromosomal deletion. (H) NOL3 mRNA normalized expression in sorted LT-HSCs from healthy control subjects (n = 4) and from patients with complex karyotype AML (n = 5). Bars represent mean values. Error bars represent ±SD; *, P < 0.05.