Table 2. Properties of fluorescent proteins constructed.
| Fluorescent protein | Excitation maximum | Emission maximum | LED excitation and filter used in imager | Filters cubes for epifluorescence microscopy | Detection settings in monochromator based plate reader | Detection settings in filter based plate reader: Ex filter/Em filter | Reference |
|---|---|---|---|---|---|---|---|
| mAmetrine | 423 | 525 | N/A | A ex: BP340-380 dichroic 400 em LP425 | 425-15/525–15 | 420–10/520EM** | 7 |
| CFP (cerulean) | 430 | 475 | N/A | CFP ET ex: BP436/20 dichroic 455 em BP480/40 | 445–15/486–15 | 420–10/485EX* | 3 |
| sGFP | 488 | 511 | EpiRGB: Blue/510DF10 GFP | GFP ET ex: BP470/40 dichroic 500 em BP525/50 | 481–15/524–15 | 485EX*/520EM** | 12 |
| YFP (venus) | 515 | 528 | EpiRGB: Blue/510DF10 GFP | YFP ET ex: BP500/20 dichroic 515 em BP535/20 | 521–15/590–70 | 485EX*/520EM** | 3 |
| mCherry | 587 | 610 | EpiRGB: Green/575DF20 Cy3 | N21 ex: BP 515–560 dichroic 580 em LP590 | 578–15/618–15 | 544EX***/590-bp10 | 3 |
| mKate2 | 588 | 633 | EpiRGB: Green/575DF20 Cy3 | N21 ex: BP 515–560 dichroic 580 em LP590 | 588–15/633–15 | 544EX***/590-bp10 | 9 |
All wavelength in nm.
Em = emission; Ex = excitation; BP = bandpass filter; LP: longpass filter,
*bp 475–490 nm; **bp 520–555 nm; ***bp 530–560 nm.