| pKTE1 |
DsRED-fw_kpnI++ |
FW |
ATCCCCGGGTACCAGGAGGAAAAACATATGGACAACA |
| DsRED-rv |
RV |
CGCGCCTGAATTCCTACAGGAACAGGTGGT |
| pJL76 |
CFP-YFP-fw_kpnI++ |
FW |
ATCCCCGGGTACCTTAGGAGGATGATTATTTATGAGTAAAG |
| CFP-YFP-rv-EcoRI |
RV |
TGACGAATTCTTACTTGTACAGCTCGTCCAT |
| pJL49 |
mCherry-FW-KpnI++_RBS |
FW |
ATCCCCGGGTACCTTAGGAGGATGTATACATATGGTGAGCAAGGGCGAGGAG |
| mCherry+Term-rv_EcoRI |
RV |
TGACGAATTCGCCTGTCACTTTGCTTGATATATGAG |
|
S.pneumoniae MK119 chromosomal DNA |
mKate-FW_KpnI++_RBS_ATG |
FW |
ATCCCCGGGTACCTTAGGAGGATGATTATTTATGTCAGAACT-TATCAAGGAAAATATGCACATGAA |
| mKate_rv-EcoRI |
RV |
TGACGAATTCTTAACGGTGTCCCAATTTACTAGG |
| 750 bp IDT-Gblock |
mAmetrine gblock FW |
FW |
ATGTATCGAGCAAGATGCATCGGATCCCCGGGTACCTT-AGGAGGATGATTATTTATGGTTTCAAAAG |
| mAmetrine gblock RV |
RV |
ATAACAATTTCACACAGGAAACAGCTATGACATGATTA-CGAATTCTTATTTATATAATTCATCCATACCTGGTGTAAT |
| pCM29/other plasmids |
pCM_PsarAfw-seq |
FW |
TTGCATGCCTGCAGGTCGACTCTA |
| pCM29-reporter-RV-seq |
RV |
TTATGCTTCCGGCTCGTATGTTGTGTGG |
|
S. aureus Newman chromosomal DNA |
NWMN_0029-FW_EcoRI |
FW |
TCACGAATTCAGTGGCTACATTCGAACATATCAA |
| NWMN_0030-RV_KpnI |
RV |
GACTGGTACCGTAAGGGTTCCGGCTTAAT |
| All replicative reporter plasmids |
pCM29_reporter_RV+ term |
RV |
ATATCGCGAGCTGCATAAAAAACGCCCGGCGGCAACCG-AGCGTTCTGAATTAACACACAGGAAACAGCTATGACATGATTA |
| pCM_reporterFW-nruI |
FW |
ATATCGCGATTGCATGCCTGCAGGTCGACTCTA |
| |
NWMN29end_FW |
FW |
TATGTCACTTATCCTTTTGGAAATG |
| |
NMWN30end_RV |
RV |
CATAATGTGTTGTAAACATTTTTTTTG |
| |
pJB38-fw_seq |
FW |
AACCTATAAAAATAGGCGTATCA |
