Figure 2. CCN1 increased IL-1β expression in primary cultured adult keratinocytes.

The mRNA (a) and protein (b) levels of IL-1β in keratinocytes stimulated with different doses of CCN1 were detected by real-time PCR and ELISA, respectively. (c) IL-1β mRNA expression in keratinocytes treated with CCN1 for 0, 2, 4, and 8 h. (d) IL-1β protein production in keratinocytes treated with CCN1 for 0, 2, 4, and 8 h. *P < 0.05, **P < 0.01. The data represent one of three independent experiments.