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. 2017 Mar 7;7:43310. doi: 10.1038/srep43310

Figure 3. CCN1 promotes mature IL-1β production in an ATP/caspase-1-dependent manner in keratinocytes.

Figure 3

(a) pro-IL-1β production in keratinocytes stimulated with CCN1 examined by western blotting. (b) The level of ATP in keratinocytes determined by an ATP bioluminescence assay kit. (c) IL-1β protein production in CCN1-treated keratinocytes after YVAD (1 μM, a caspase 1 inhibitor) treatment. For (b,c), 5 μg/ml of CCN1 (black bar) or BSA (open bar) was used to stimulate keratinocytes. *P < 0.05. The data represent one of three independent experiments.