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. 2004 Dec;24(24):10718–10732. doi: 10.1128/MCB.24.24.10718-10732.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 4. — Identification of FAST/TIA1 interaction sites. (A) Schematic depiction of HA-FASTN and HA-FASTC truncation mutants. MTD, mitochondrial tethering domain; BH3, BCL2 homology 3-related domain. (B) Schematic depiction of HA-TIA-1 truncation mutants. 1, 2, 3, RNA-recognition motifs; PRD, prion-related domain. (C) COS-7 cells were cotransfected with pcDNA3-FLAG-TIA1 and the indicated HA-FAST truncation mutants. After 28 h, cells were processed for coimmunoprecipitation analysis with mouse anti-HA antibody followed by Western blotting analysis with mouse anti-FLAG antibody, followed by mouse anti-HA antibody. Lysates from transfected cells were analyzed by Western blotting with anti-FLAG antibody (load). (D) COS-7 cells were cotransfected with pcDNA3-FLAG-FAST and the indicated HA-TIA1 truncation mutants. Lysates from transfected cells were immunoprecipitated with anti-HA antibody and then were sequentially blotted with anti-FLAG antibody and anti-HA antibody. Lysates from transfected cells were analyzed by Western blotting with anti-FLAG antibody (load). FL, FLAG.