FAST enhances the expression of cotransfected β-galactosidase. The left panel shows COS-7 cells cotransfected with a β-galactosidase reporter together with pMT2 alone, pMT2-HA-FAST, pMT2-HA-TIA-1, or pMT2-HA-TIA-1-PRD. After 48 h, cells were processed for Western blotting analysis to quantify the expression of β-galactosidase (β-gal). The right panel shows COS-7 cells cotransfected with a β-galactosidase reporter together with pMT2-HA-FAST and increasing amounts of pMT2-HA-TIA-1 (5:1, 2:1, 1:1). After 48 h, cells were processed for Western blotting analysis to quantify the expression of β-galactosidase and the expression of recombinant TIA-1 and FAST.