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. 2017 Mar 7;7:43210. doi: 10.1038/srep43210

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Copyright © 2017, The Author(s)

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(a) Field potential features such as beat interval, field potential duration, amplitude, and local activation time (LAT) were extracted out of the signal sequences. On average, 100 out of 120 channels were selected to calculate the beat interval after the peak detection and experiments were performed in triplicate. (b) The beat interval was stable up to day 28–30 with a beat interval around 2 s. Starting from day 28, the beat interval of hESC-CMs became unstable and irregular. The standard deviation (δ) of beat interval agreed with the beat interval trend. (c) The field potential duration (FPD) ranged from 0.2 s to 0.5 s and was relatively stable throughout the differentiation process. (d) The corrected FPD ( = FPD/[beat interval/1000]^1/3; Fridericia’s formula) was also relatively stable ranging from 0.2 to 0.3.