FIGURE 2.

Quininib analogues reduce endothelial cell number after 24 h and inhibit endothelial cell migration. A, effect of 10 μm quininib analogues on HMEC-1 endothelial cell number using the MTT assay following 24-, 72-, and 96-h treatment. 10,000 cells were seeded and treated in duplicate for each individual experiment, and individual experiments were conducted three times (n = 3). B, effect of 10 μm quininib analogues on HMEC-1 endothelial cell migration. 50,000 cells were seeded and treated in duplicate for each individual experiment, and individual experiments were conducted three times (n = 3). C, effect of Q8 in combination with bevacizumab on HMEC-1 endothelial cell migration. 50,000 cells were seeded and treated in duplicate for each individual experiment, and individual experiments were conducted three times (n = 3). Migration was assessed using the xCELLigence system and RTCA software allowing real time monitoring of cell migration over 8 h. The real time traces (B and C, right) represent averaged data of all detected/bound cells from 0 to 8 h. Statistical analysis was performed by ANOVA, Dunnett's post hoc multiple comparison test, and Student's t test. Error bars are mean ± S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.