TABLE 1.
Localized injection of MB caused various developmental defects
| Developmental defectsa |
||||
|---|---|---|---|---|
| Brain/notochord | Heart | Kidneyb | Blood/circulationc | |
| True positived | 7/10 (70%) | 17/18 (95%) | 13/16 (81%) | 10/12 (83%) |
| False positivee | 0/13 (0%) | 2/5 (40%) | 4/7 (57%) | 1/11 (9%) |
a Zebrafish embryos were injected with MB locally and monitored for developmental defects from 1 to 7 dpf as described in Fig. 5.
b Defect in kidney function was scored by total body edema as shown in Fig. 5C.
c Defect in blood/circulation was scored by the complete absence of blood and movement in the circulation system when viewed under light microscope.
d True positive means embryos scored with developmental defects matched with the accumulation of MB in the specification region.
e False positive means embryos scored with developmental defects not matched with the accumulation of MB in the specification region.