TABLE 2.

Effect of MB injection on the expression of genes regulated by miR-430b

Genes	ΔCTa,b		ΔΔCT	Relative quantity (compared with control)c
	MB	Control
Mknk2b	1.38 ± 1.6	3.89 ± 0.16	−2.52 ± 1.58	5.74 (1.90–17.1)
Got2a	6.38 ± 0.48	7.69 ± 0.34	−1.31 ± 0.48	2.48 (1.77–3.46)
CTZS	5.86 ± 0.69	7.25 ± 0.07	−1.40 ± 0.69	2.63 (1.63–4.24)

^a Embryos were either left untreated (control) or injected with MB (0.02 pmol) at the 1-cell stage. At 48 hpf, embryos were collected in RNAlater for subsequent RNA extraction and quantification of gene expression by qPCR.

^b Three to four biological replicates, with 25–30 embryos/replicate, were used to determine averaged ΔC_T ± S.D. using actin as the reference gene.

^c Fold change in gene expression relative to control was determined by 2^−ΔΔCT with the range indicated in parethenses.