FIGURE 6.

YTHDF1 had no effect on m⁶A levels of mRNAs. A, levels of m⁶A were measured by dot blot assays in HepG2 cells transfected with pcDNA3.1-YTHDF1. B, quantification of m⁶A in A. C, immunofluorescence images of m⁶A (red) are shown. DAPI staining (blue) was included to visualize the nucleus. D, quantification of the intensity of fluorescence per cell of m⁶A levels in C. E, levels of m⁶A were measured by dot blot assays in HepG2 cells transfected with si-YTHDF1. F, quantification of m⁶A in E. G, immunofluorescence images of m⁶A (red) are shown. DAPI staining (blue) was included to visualize the nucleus. H, quantification of the intensity of fluorescence per cell of m⁶A levels in G. NS, not significant.