FIGURE 7.

miR-145 targets YTHDF2 and suppresses proliferation of HCC cells. A, HepG2 cells were transfected with miRNA control, miR-145, anti-miR-145, and YTHDF2 siRNA. B, interference efficiency of YTHDF2 siRNA was validated by Western blot analysis in HepG2 cells. The effect of miR-145 on cell proliferation was determined by MTT assays. Statistically significant differences are indicated: **, p < 0.01; Student's t test. C, a sketch of the mechanism by which YTHDF2 regulates m⁶A levels of mRNAs. D, a model shows that miR-145 increases m⁶A levels of mRNAs through down-regulating YTHDF2.