TABLE 2.

Sequencing primers used to verify correct sequences of engineered second site mutants of CBL-Y371H

Fwd means forward, and Rev means reverse.

CBL sequencing primers
Primer	Sequence (5′–3′)	Start site
Fwd 1	TGTTTATGGAGAATTTGATGAAGAA	350
Fwd 2	CCTCTTTGCTCAGGAATTGG	755
Fwd 3	TGTGCTGAAAATGATAAGGATG	1150
Fwd 4	TCTAAGGCTGCTTCTGGCTC	1558
Fwd 5	GCAGCCCATTAGTAGGTCCA	1952
Fwd 6	CCGCCGAACTCTCTCAGAT	2358
Rev 1	TTCGCCTAGGCTGAGAAT	448
Rev 2	CCGAGCTTTCACTTCGTCAT	840
Rev 3	CTGACCTTCTGATTCCTGCC	1242
Rev 4	AGGCGGTGGTGGTGGAAG	1641
Rev 5	CACAGGAAGAGGTCTGGCA	2049
Rev 6	TGACATTTGTTGTAGGATCACCA	2440