TABLE 1.
Data collection and refinement statistics for E. coli ZapD-FtsZ CTD structure
| Data collection | |
| Space group | P21 |
| Cell constants (Å) | a = 71.3, b = 51.9, c = 79.1 |
| β = 90.7 | |
| Resolution (Å) | 79.1–2.67 |
| Rsym(%)a | 7.5 (48.0)b |
| Rpim (%) | 5.0 (40.9) |
| Overall I/σ(I) | 10.7 (1.6) |
| No. of unique reflections | 15,706 |
| No. of total reflections | 47,044 |
| % complete | 94.1 (73.1) |
| CC(1/2) | 0.997 (0.716) |
| Refinement statistics | |
| Rwork/Rfree(%)c | 23.0/26.5 |
| MolProbity score | 2.11 |
| B-Factors (average) (Å2) | |
| ZapD | 48.4 |
| CTD peptide | 80.0/69.2d |
| Solvent | 38.0 |
| r.m.s.d. | |
| Bond angles (°) | 0.445 |
| Bond lengths (Å) | 0.002 |
| Ramachandran analysis | |
| Favored (%) | 94.6 |
| Disallowed (%) | 0.0 |
aRsym = ΣΣ|Ihkl − Ihkl(j)|/ΣIhkl, where Ihkl(j) is observed intensity and Ihkl is the final average value of intensity.
bValues in parentheses are for the highest resolution shell.
cRwork = Σ‖Fobs| − |Fcalc‖/Σ|Fobs| and Rfree = Σ‖Fobs| − |Fcalc‖/Σ|Fobs|, where all reflections belong to a test set of 5% randomly selected data.
dB-factor (average) for all CTD residues/B-factor (average) for CTD residues minus disordered side chains of residues 270, 381.