TABLE 2.
Bacterial strains and plasmid used in this study
| Strains and plasmids | Relevant genotype | Source or Ref.a |
|---|---|---|
| Strains | ||
| MG1655 | F− λ− ilvG rfb50 rph1 | Laboratory collection |
| C41 (DE3) | F− ompT hsdSB (rB− mB−) gal dcm (DE3) | 49 |
| KHH72 | C41 (DE3) pET28b-h10-smt3-zapD | |
| KHH441 | MG1655 pTrc99a | |
| KHH442 | MG1655 pTrc99a-zapD | |
| KHH443 | MG1655 pTrc99a-zapD D84A | |
| KHH445 | MG1655 pTrc99a-zapD L91A | |
| KHH448 | MG1655 pTrc99a-zapD (W77A) | |
| KHH478 | MG1655 pTrc99a-zapD (L174A) | |
| KHH488 | C41 (DE3) pET28b-h10-smt3-zapD (L174A) | |
| KHH490 | C41 (DE3) pET21b-ftsZ | |
| KHH491 | C41 (DE3) pET28b-h10-smt3-zapD (W77A) | |
| KHH492 | C41/DE3 pET28b-h10-smt3-zapD (L91A) | |
| Plasmids | ||
| pAM4 | pTrc99a-zapD (L174A) | |
| pET28b-his10-smt3-zapD | pBR322 ori PT7 h10-smt3-zapD, KanR | 20 |
| pKHH45 | pTrc99a zapD (W77A) | |
| pKHH46 | pTrc99a zapD (D84A) | |
| pKHH47 | pTrc99a zapD (L91A) | |
| pKHH48 | pET28b h10-smt3-zapD (L174A) | |
| pKHH49 | pET28b h10-smt3-zapD (L91A) | |
| pKHH50 | pET28b h10-smt3-zapD (W77A) | |
| pTrc99a | ColE1 ori Ptac AmpR | Laboratory collection |
a This work was used unless otherwise noted.