Skip to main content
. 2017 Jan 18;292(9):3740–3750. doi: 10.1074/jbc.M116.773192

TABLE 3.

Primers used in this study

F is forward, and R is reverse.

Name Sequence 5′–3′ Plasmid
SUMO-5 YacF-BamHI CGCGGATCCATGCAGACCCAGGTCCTTTTTG pET28b-his10x-smt3-zapD
SUMO-3 YacF-HindIII CCCAAGCTTTTAGCAACAGGCCAGTTCGAA pET28b-his10×-smt3-zapD
ZapD-BamHI F GGTAGGATCCATGCAGACCCAGGTCCTTTTTGA pKHH48–50
ZapD-SalI R GGCGGTCGACTTAGCAACAGGCCAGTTCGAAAT pAM4, pKHH44–50
ZapD-W77A F GCTAAACTCCAGACCGCGATTGGCGTGCCTGG pKHH45
ZapD-W77A R CCAGGCACGCCAATCGCGGTCTGGAGTTTAGC pKHH45
ZapD-D84A F CGTGCCTGGCGTGGCCCAGAGCCGTATTGA pKHH46
ZapD-D84A R TCAATACGGCTCTGGGCCACGCCAGGCACG pKHH46
ZapD-L91A F CCGTATTGAAGCAGCAATTCAGCAGTTAAA pKHH47
ZapD-L91A R TTTAACTGCTGAATTGCAGCTTCAATACGG pKHH47
ZapD-L174A F CATGGTGCTGGATGCAATTCGCCAGTCGGC pAM4
ZapD-L174A R CGGCACTGGCGAATTGCATCCAGCTCCATG pAM4