SUMO-5 YacF-BamHI |
CGCGGATCCATGCAGACCCAGGTCCTTTTTG |
pET28b-his10x-smt3-zapD
|
SUMO-3 YacF-HindIII |
CCCAAGCTTTTAGCAACAGGCCAGTTCGAA |
pET28b-his10×-smt3-zapD
|
ZapD-BamHI F |
GGTAGGATCCATGCAGACCCAGGTCCTTTTTGA |
pKHH48–50 |
ZapD-SalI R |
GGCGGTCGACTTAGCAACAGGCCAGTTCGAAAT |
pAM4, pKHH44–50 |
ZapD-W77A F |
GCTAAACTCCAGACCGCGATTGGCGTGCCTGG |
pKHH45 |
ZapD-W77A R |
CCAGGCACGCCAATCGCGGTCTGGAGTTTAGC |
pKHH45 |
ZapD-D84A F |
CGTGCCTGGCGTGGCCCAGAGCCGTATTGA |
pKHH46 |
ZapD-D84A R |
TCAATACGGCTCTGGGCCACGCCAGGCACG |
pKHH46 |
ZapD-L91A F |
CCGTATTGAAGCAGCAATTCAGCAGTTAAA |
pKHH47 |
ZapD-L91A R |
TTTAACTGCTGAATTGCAGCTTCAATACGG |
pKHH47 |
ZapD-L174A F |
CATGGTGCTGGATGCAATTCGCCAGTCGGC |
pAM4 |
ZapD-L174A R |
CGGCACTGGCGAATTGCATCCAGCTCCATG |
pAM4 |