TABLE 4.

Cell lengths of strains overexpressing either WT ZapD or a ZapD mutant in trans

Plasmida	Average ± S.D.b	Nc
	μm
Vector	3.0 ± 0.7	331
ZapD	46.6 ± 17.9	71
W77A	6.4 ± 3.3	278
D84A	21.2 ± 13.7	102
L91A	7.0 ± 4.8	252
L174A	3.4 ± 1.1	374

^a Wild type or ZapD mutant plasmids were expressed in trans in the pTrc99a vector backbone in a wild type strain (MG1655) background.

^b Cell lengths reported are for cells sampled at OD = ∼0.6–0.8 grown in LB with 25 μm IPTG at 37 °C. S.D. = standard deviation.

^c N represents the number of individual cells measured for each strain. In case of filamentous strains, fewer numbers of cells were present within each field of view.