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. 2004 Dec;24(24):10826–10834. doi: 10.1128/MCB.24.24.10826-10834.2004

FIG. 1.

FIG. 1.

The c-MYC oncoprotein is acetylated in vivo by its cofactors mGCN5 and TIP60. (A) The human lung cancer cell line H1299 was transfected with an expression vector for c-MYC (lanes 2 to 4). Mock transfected cells served as a negative control (lane 1). In addition, transfections included expression vectors for the acetyltransferases mGCN5 and TIP60, as indicated. Twenty-four hours posttransfection, cell lysates were produced, and acetylated proteins were precipitated with a universal antiacetyllysine antibody. Precipitates (top panel) and lysates (bottom panel) were resolved by SDS-PAGE and Western blotted for c-MYC. (B) H1299 cells were transfected with expression vectors for mGCN5 or TIP60, as indicated. Lysates were subjected to immunoprecipitation for the FLAG epitope present on the acetyltransferases. Precipitates were either resolved by SDS-PAGE and Western blotted for the FLAG epitope (top panel) or subjected to an in vitro acetyltransferase assay using purified histones as the substrate (bottom panel). The migration of molecular weight markers is indicated at the left. The migration of the immunoglobulin heavy chain from the immunoprecipitating antibody is indicated (Ig).